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Location： Home > KD/KO Validation > Raf1 (Phospho-Ser621) Rabbit mAb

Raf1 (Phospho-Ser621) Rabbit mAb#14227

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Product NameRaf1 (Phospho-Ser621) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Raf1 (S621) Antibody detects endogenous levels of total Phospho-Raf1 (S621)

Immunogen DescA synthesized peptide derived from human Phospho-Raf1 (S621)

ConjugateUnconjugated

Other NamesC-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

Accession NoUniprot:P04049

Uniprot P04049

Calculated MW73kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:2000
All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution

Lane 1 : Hela treated with alkaline phosphatase whole cell lysates
Lane 2 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 7 seconds
All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 10 seconds
All lanes: Raf1 (Phospho-Ser621) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Raf1 (Phospho-Ser621) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 14227, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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