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Raf1 (Phospho-Ser259) Rabbit mAb#14228

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Product Detail

Product NameRaf1 (Phospho-Ser259) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Raf1 (S259) Antibody detects endogenous levels of total Phospho-Raf1 (S259)

Immunogen DescA synthesized peptide derived from human Phospho-Raf1 (S259)

ConjugateUnconjugated

Other NamesC-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

Accession NoUniprot:P04049

Uniprot P04049

Calculated MW73kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:1000
IHC:1:50~1:100
Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.

If you have published an article using product 14228, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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