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B Raf (Phospho-Thr401) Rabbit mAb#14231

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Product Detail

Product NameB Raf (Phospho-Thr401) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityPhospho-B Raf (T401) Antibody detects endogenous levels of total Phospho-B Raf (T401)

Immunogen DescA synthesized peptide derived from human Phospho-B Raf (T401)

ConjugateUnconjugated

Other NamesB-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; BRAF1; RAFB1; RMIL; RMIL serine/threonine-protein kinase; c-RMIL; kinase B-Raf;

Accession NoUniprot:P15056

Uniprot P15056

Calculated MW85kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:2000
ICC/IF:1:50~1:200
IP:1:30
Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA.

If you have published an article using product 14231, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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