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Raf1 (Phospho-Ser43) Rabbit mAb#14232

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Product Detail

Product NameRaf1 (Phospho-Ser43) Rabbit mAb

Clone No.SR2182

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman;Mouse;Rat

SpecificityPhospho-Raf1 (S43) Antibody detects endogenous levels of total Phospho-Raf1 (S43)

Immunogen DescA synthesized peptide derived from human Phospho-Raf1 (S43)

ConjugateUnconjugated

Other NamesC-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

Accession NoUniprot:P04049

Uniprot P04049

Calculated MW73 kDa

Sdspage MW65-75 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:1000

All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution

Lane 1 : MCF7 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : 293T whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 65-75 kDa

Exposure time: 10 seconds
All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution

Lane 1 : 3T3 whole cell lysates
Lane 2 : Mouse Heart lysates
Lane 3 : Rat Brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 65-75 kDa

Exposure time: 10 seconds
All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution

Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 treated with 200nM PMA for 30min whole cell lysates
Lane 3 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 65-75 kDa

Exposure time: 7 seconds

If you have published an article using product 14232, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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