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beta Catenin (Phospho-Ser33/Ser37) Rabbit mAb#14233

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Product Namebeta Catenin (Phospho-Ser33/Ser37) Rabbit mAb

Clone No.SR2294

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Rat

SpecificityPhospho-beta Catenin (S33/S37) Antibody detects endogenous levels of total Phospho-beta Catenin (S33/S37)

Immunogen DescA synthesized peptide derived from human Phospho-beta Catenin (S33/S37)

ConjugateUnconjugated

Other NamesCATNB; CTNB1; CTNNB; catenin beta; beta catenin;

Accession NoUniprot:P35222

Uniprot P35222

Calculated MWPredicted band size: 85 kDa

Sdspage MWObserved band size: 92 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : beta Catenin (Phospho-Ser33/Ser37) Rabbit mAb at 1/1k dilution
Lane 1 :293T cell lysate
Lane 2 : 293T treated with 100nM Calyculin A for 30min whole cell lysates
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 92 kDa
Exposure time: 9 seconds
All lanes : beta Catenin (Phospho-Ser33/Ser37) Rabbit mAb at 1/1k dilution
Lane 1 :Rat brain lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 92 kDa
Exposure time: 4 seconds

If you have published an article using product 14233, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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