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KD/KO Validation > GSK3β(Phospho-Ser9) Rabbit mAb
GSK3β(Phospho-Ser9) Rabbit mAb#14237
Yes
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 46 kDa
Exposure time: 7 seconds
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution
Lane 1 : 293T whole cell lysates
Lane 2 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 46 kDa
Exposure time: 3 seconds
All lanes : Phospho-GSK3 beta (S9) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Phospho-GSK3 beta (S9) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Phospho-GSK3 beta (S9) using 14237 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence p-GSK3 beta (S9) antibody (14237)
14237 staining p-GSK3 beta (S9) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14237 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control, purified 14237 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
