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Phospho Antibodies > Phosphotyrosine Rabbit mAb
Phosphotyrosine Rabbit mAb#14247
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All lanes : Phosphotyrosine Rabbit mAb at 1/1k dilutionLane 1 : Lane 1 :Western blot analysis of Phosphotyrosine expression in Jurkat cell lysate.Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionExposure time: 5 seconds
Immunocytochemistry/Immunofluorescence Phosphotyrosine antibody (14247)
ICC/IF staining of Phosphotyrosine in A431 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14247 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
