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Location： Home > Phospho Antibodies > MLKL (Phospho-Ser358) Rabbit mAb

MLKL (Phospho-Ser358) Rabbit mAb#14251

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Product NameMLKL (Phospho-Ser358) Rabbit mAb

Clone No.SR1963

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman

SpecificityPhospho-MLKL (S358) Antibody detects endogenous levels of total Phospho-MLKL (S358)

Immunogen DescA synthesized peptide derived from human Phospho-MLKL (S358)

ConjugateUnconjugated

Other NameshMLKL; Mlkl;

Accession NoUniprot:Q8NB16

Uniprot Q8NB16

Calculated MWPredicted band size: 54 kDa

Sdspage MWObserved band size: 54 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : MLKL (Phospho-Ser358) Rabbit mAb at 1/1k dilution
Lane 1 :HT-29 cell lysate
Lane 2 : HT-29 cell lysate treated with 20 ng/ml TNF-a, 100 nM SM-164 and 20 µM z-VAD
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 9 seconds
Formalin-fixed, paraffin-embedded human small intestinal carcinoma tissue stained for MLKL (Phospho-Ser358) using 14251 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14251, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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