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ERK1/2(Phospho-Thr202/Thr185) Rabbit mAb#14253

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Product Detail

Product NameERK1/2(Phospho-Thr202/Thr185) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityPhospho-ERK1 (T202) + ERK2 (T185) Antibody detects endogenous levels of total Phospho-ERK1 (T202) + ERK2 (T185)

Immunogen DescA synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)

ConjugateUnconjugated

Other NamesMAPK3; MK03; MNK1; p44-ERK1; P44-ER; ERK-1; ERK1; ERT2; kinase ERK1; MAP kinase 1; MAPK 1;

Accession NoUniprot:P27361

Uniprot P27361

Calculated MW42,44kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:1000
IHC:1:50~1:200
ICC/IF:1:50~1:200
IP:1:40
Western blot analysis of Phospho-ERK1 (T202) + ERK2 (T185) expression in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.

If you have published an article using product 14253, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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