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KAP1 (Phospho-Ser824) Rabbit mAb#14256

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Product Detail

Product NameKAP1 (Phospho-Ser824) Rabbit mAb

Clone No.SR1057

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman

SpecificityPhospho-KAP1 (S824) Antibody detects endogenous levels of total Phospho-KAP1 (S824)

Immunogen DescA synthesized peptide derived from human KAP1

ConjugateUnconjugated

Other NamesKAP 1; KRAB associated protein 1; KRIP1; RNF96; TF1B; TIF1 beta; TIF1B; Trim28; Tripartite motif containing 28;

Accession NoUniprot:Q13263

Uniprot Q13263

Calculated MWPredicted band size: 89 kDa

Sdspage MWObserved band size: 110 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : KAP1 (Phospho-Ser824) Rabbit mAb at 1/1k dilution
Lane 1 :HeLa cell lysate
Lane 2 : HeLa treated with Etoposide cell lysate
Lysates/proteins at 20 µg per lane.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 110 kDa
Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for KAP1 (Phospho-Ser824) using 14256 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14256, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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