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Location： Home > Phospho Antibodies > ErbB3 (Phospho-Tyr1222) Rabbit mAb

ErbB3 (Phospho-Tyr1222) Rabbit mAb#14258

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Product NameErbB3 (Phospho-Tyr1222) Rabbit mAb

Clone No.SR1278

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF

Species ReactivityHuman

SpecificityPhospho-ErbB3 (Y1222) Antibody detects endogenous levels of total Phospho-ErbB3 (Y1222)

Immunogen DescA synthesized peptide derived from human ErbB3

ConjugateUnconjugated

Other NamesERBB3; HER3; LCCS2; p180 ErbB3; p45 sErbB3; p85 sErbB3;

Accession NoUniprot:P21860

Uniprot P21860

Calculated MWPredicted band size: 148 kDa

Sdspage MWObserved band size: 185 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : ErbB3 (Phospho-Tyr1222) Rabbit mAb at 1/1k dilution
Lane 1 :SKBR3 cell
Lane 2 : SKBR3 cell treated with neuregulin
Lysates/proteins at 20 µg per lane.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 148 kDa
Observed band size: 185 kDa
Exposure time: 9 seconds
Immunocytochemistry/Immunofluorescence ErbB3 (Phospho-Tyr1222) antibody (14258)

ICC/IF staining of ErbB3 (Phospho-Tyr1222) in HUVEC cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14258 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14258, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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