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Location： Home > Phospho Antibodies > ErbB2 (Phospho-Tyr1139) Rabbit mAb

ErbB2 (Phospho-Tyr1139) Rabbit mAb#14263

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Product Detail

Product NameErbB2 (Phospho-Tyr1139) Rabbit mAb

Clone No.SR1274

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityPhospho-ErbB2 (Y1139) Antibody detects endogenous levels of total Phospho-ErbB2 (Y1139)

Immunogen DescA synthesized peptide derived from human Phospho-ErbB2 (Y1139)

ConjugateUnconjugated

Other NamesCD340; CerbB2; ERBB2; HER2; Herstatin; MLN19; NEU; NGL; p185erbB2; TKR1;

Accession NoUniprot:P04626

Uniprot P04626

Calculated MWPredicted band size: 138 kDa

Sdspage MWObserved band size: 180 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : ErbB2 (Phospho-Tyr1139) Rabbit mAb at 1/1k dilution
Lane 1 :A431 cell lysate
Lane 2 : A431 treated with 100ng/ml EGF 10min
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 13 seconds

If you have published an article using product 14263, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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