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MLKL (Phospho-Ser345) Rabbit mAb#14264

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Product NameMLKL (Phospho-Ser345) Rabbit mAb

Clone No.SR1962

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityMouse

SpecificityPhospho-MLKL (S345) Antibody detects endogenous levels of total Phospho-MLKL (S345)

Immunogen DescA synthesized peptide derived from human Phospho-MLKL (S345)

ConjugateUnconjugated

Other NameshMLKL ; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase;

Accession NoUniprot:Q9D2Y4

Uniprot Q9D2Y4

Calculated MWPredicted band size: 54 kDa

Sdspage MWObserved band size: 54 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : MLKL (Phospho-Ser345) Rabbit mAb at 1/1k dilution
Lane 1 :L929 cell lysate
Lane 2 : L929 cell lysate treated with 20 ng/ml TNF-a, 100 nM SM-164 and 20 µM z-VAD
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded mouse colon tissue stained for MLKL (Phospho-Ser345) using 14264 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14264, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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