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Location： Home > Phospho Antibodies > TAK1(Phospho-Ser439) Rabbit mAb

TAK1(Phospho-Ser439) Rabbit mAb#14266

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Product Detail

Product NameTAK1(Phospho-Ser439) Rabbit mAb

Clone No.SR2176

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-TAK1 (S439) Antibody detects endogenous levels of total Phospho-TAK1 (S439)

Immunogen DescA synthesized peptide derived from human Phospho-TAK1 (S439)

ConjugateUnconjugated

Other NamesMAP3K 7; MEKK7; Mitogen activated protein kinase kinase kinase 7; TAK1; TGF beta activated kinase 1; TGF1a;

Accession NoUniprot:O43318

Uniprot O43318

Calculated MWPredicted band size: 67 kDa

Sdspage MWObserved band size: 72 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes :TAK1(Phospho-Ser439) Rabbit mAb at 1/1k dilution
Lane 1 :K562 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 72 kDa

Exposure time: 13 seconds
All lanes :TAK1(Phospho-Ser439) Rabbit mAb at 1/1k dilution
Lane 1 :HeLa treated with Calyculin A + IL-1 beta cell lysate
Lane 2 : Untreated lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 72 kDa

Exposure time: 9 seconds

If you have published an article using product 14266, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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