Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Rad17(Phospho-Ser656) Rabbit mAb

Rad17(Phospho-Ser656) Rabbit mAb#14271

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameRad17(Phospho-Ser656) Rabbit mAb

Clone No.SR2259

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman

SpecificityPhospho-Rad17 (S656) Antibody detects endogenous levels of total Phospho-Rad17 (S656)

Immunogen DescA synthesized peptide derived from human Phospho-Rad17 (S656)

ConjugateUnconjugated

Other NamesCCYC; hRad17; R24L; RAD17; Rad24;

Accession NoUniprot:O75943

Uniprot O75943

Calculated MWPredicted band size: 77 kDa

Sdspage MWObserved band size: 85 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes :Rad17(Phospho-Ser656) Rabbit mAb at 1/1k dilution
Lane 1 :HeLa cell treated with CA lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 85 kDa

Exposure time: 13 seconds
Formalin-fixed, paraffin-embedded human lung tissue stained for Rad17(Phospho-Ser656) using 14271 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence Rad17(Phospho-Ser656) antibody (14271)

ICC/IF staining of Rad17(Phospho-Ser656) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14271 at a working dilution of 1/100.Goat Anti Rabbit Alexa Fluor 647 was diluted at 1:1000.
Nuclei were counterstained with DAPI.

If you have published an article using product 14271, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors