Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Nucleolin (Phospho-Thr76) Rabbit mAb

Nucleolin (Phospho-Thr76) Rabbit mAb#14280

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameNucleolin (Phospho-Thr76) Rabbit mAb

Clone No.SR1019

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman

SpecificityPhospho-Nucleolin (T76) Antibody detects endogenous levels of total Phospho-Nucleolin (T76)

Immunogen DescA synthesized peptide derived from human Phospho-Nucleolin (T76)

ConjugateUnconjugated

Other NamesMS1116; NCL; Nucl; Nucleolin; Protein C23;

Accession NoUniprot:P19338

Uniprot P19338

Calculated MWPredicted band size: 77 kDa

Sdspage MWObserved band size: 100 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes :Nucleolin (Phospho-Thr76) Rabbit mAb at 1/1k dilution
Lane 1 :293T cell lysate
Lane 2 : 293T treated with Lambda phosphatase for 30min cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100 kDa

Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded human kidney tissue stained for Nucleolin (Phospho-Thr76) using 14280 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14280, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors