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PBK/TOPK (Phospho-Thr9) Rabbit mAb#14283

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Product Detail

Product NamePBK/TOPK (Phospho-Thr9) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman

SpecificityPhospho-PBK/TOPK (Thr9) Antibody detects endogenous levels of total Phospho-PBK/TOPK (Thr9)

Immunogen DescA synthesized peptide derived from human Phospho-PBK/TOPK (Thr9)

ConjugateUnconjugated

Other NamesCT84; MAPKK like protein kinase; Nori3; PBK; PDZ binding kinas; Serine/threonine protein kinase; SPK; TOPK;

Accession NoUniprot:Q96KB5

Uniprot Q96KB5

Calculated MW40kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200

All lanes: PBK/TOPK (Phospho-Thr9) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/ml Nocodazole for 16h whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 40 kDa

Exposure time: 6 seconds
All lanes:PBK/TOPK (Phospho-Thr9) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : PBK/TOPK (Phospho-Thr9) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 14283, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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