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Phospho Antibodies > c-Jun/JunD(Phospho-Ser73+Ser100) Rabbit mAb
c-Jun/JunD(Phospho-Ser73+Ser100) Rabbit mAb#14284
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All lanes : c-Jun/JunD(Phospho-Ser73+Ser100) Rabbit mAb at 1/1k dilutionLane 1 :HeLa cell lysateLane 2 : HeLa treated with 5ug/mL Anisomycin for 30 minutes cell lysateLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 36 kDa Observed band size: 38-40 kDaExposure time: 4 seconds
All lanes : c-Jun/JunD(Phospho-Ser73+Ser100) at 1/1k dilutionLane 1 :NIH/3T3 cell lysateLane 2 : NIH/3T3 treated with 25ug/mL Anisomycin for 30 minutes cell lysateLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 36 kDa Observed band size: 38-40 kDaExposure time: 5 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Jun/JunD(Phospho-Ser73/Ser100)using 14284 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence c-Jun/JunD(Phospho-Ser73/Ser100) antibody (14284)
ICC/IF staining of c-Jun/JunD(Phospho-Ser73/Ser100) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14284 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
