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AS160 (Phospho-Thr642) Rabbit mAb#14291

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Product NameAS160 (Phospho-Thr642) Rabbit mAb

Clone No.SR1272

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman

SpecificityPhospho-AS160 (T642) Antibody detects endogenous levels of total Phospho-AS160 (T642)

Immunogen Desc A synthesized peptide derived from human Phospho-AS160 (T642)

ConjugateUnconjugated

Accession NoO60343

Uniprot O60343

Calculated MWPredicted band size: 147 kDa

Sdspage MWObserved band size: 180 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : AS160 (Phospho-Thr642) Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates
Lane 2 : 293 treated with 100nM Calyculin A for 30min and then 100ng/ml Insulin was added for the last 20min ,whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 180 kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded human lung tissue stained for AS160 (Phospho-Thr642) using 14291 at 1/100 dilution in immunohistochemical analysis.

  May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

If you have published an article using product 14291, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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