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Chk1 (Phospho-Ser280) Rabbit mAb#14293

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Product Detail

Product NameChk1 (Phospho-Ser280) Rabbit mAb

Clone No.SR1952

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityPhospho-Chk1 (S280) Antibody detects endogenous levels of total Phospho-Chk1 (S280)

Immunogen Desc A synthesized peptide derived from human Phospho-Chk1 (S280)

ConjugateUnconjugated

Other NamesCheckpoint kinase 1; Chek1; Chk1; rad27;

Accession NoO14757

Uniprot O14757

Calculated MWPredicted band size: 54 kDa

Sdspage MWObserved band size: 54 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
All lanes : Chk1 (Phospho-Ser280) Rabbit mAb at 1/1k dilution
Lane 1 :293T whole cell lysates
Lane 2 : 293T treated with 100nM Calyculin A for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 seconds

  Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles.

If you have published an article using product 14293, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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