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Location： Home > Phospho Antibodies > EGFR (Phospho-Tyr869) Rabbit mAb

EGFR (Phospho-Tyr869) Rabbit mAb#14294

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Product NameEGFR (Phospho-Tyr869) Rabbit mAb

Clone No.SR1249

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityPhospho-EGFR (Y845) Antibody detects endogenous levels of total Phospho-EGFR (Y845)

Immunogen Desc A synthesized peptide derived from human Phospho-EGFR (Y845)

ConjugateUnconjugated

Other NamesEGFR; ERBB; ERBB1; Errp; HER1; mENA; NISBD2; PIG61; SA7; wa2; Wa5;

Accession NoP00533

Uniprot P00533

Calculated MWPredicted band size: 135 kDa

Sdspage MWObserved band size: 175 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
All lanes : EGFR (Phospho-Tyr869) Rabbit mAb at 1/1k dilution
Lane 1 :A431 cells treated with EGF 100ng/ml 10'
Lane 2 : blocked with the phospho peptide
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 175 kDa
Exposure time: 8 seconds

  Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

If you have published an article using product 14294, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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