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RIP3 (Phospho-Ser232) Rabbit mAb#14299

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Product Detail

Product NameRIP3 (Phospho-Ser232) Rabbit mAb

Clone No.SR1864

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityMouse

SpecificityPhospho-RIP3 (S232) Antibody detects endogenous levels of total Phospho-RIP3 (S232)

Immunogen Desc A synthesized peptide derived from human Phospho-RIP3 (S232)

ConjugateUnconjugated

Other NamesReceptor interacting protein 3; Receptor interacting serine threonine kinase 3; Receptor interacting serine/threonine protein kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP like protein kinase 3; RIP-3;RIP-like protein kinase 3;

Accession NoQ9Y572/Q9QZL0

Uniprot Q9Y572/Q9QZL0

Calculated MWPredicted band size: 53 kDa

Sdspage MWObserved band size: 50 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
All lanes : RIP3 (Phospho-Ser232) Rabbit mAb at 1/1k dilution
Lane 1 :L929 cell lysate
Lane 2 : L929 cell lysate treated with 20 ng/ml TNF-a, 100 nM SM-164 and 20 µM z-VAD
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Exposure time: 4 seconds

   Promotes apoptosis.

If you have published an article using product 14299, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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