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Location： Home > Phospho Antibodies > ULK1 (Phospho-Ser556) Rabbit mAb

ULK1 (Phospho-Ser556) Rabbit mAb#14300

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Product Detail

Product NameULK1 (Phospho-Ser556) Rabbit mAb

Clone No.SR1119

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF

Species ReactivityHuman Mouse

SpecificityPhospho-ULK1 (S556) Antibody detects endogenous levels of total Phospho-ULK1 (S556)

Immunogen Desc A synthesized peptide derived from human Phospho-ULK1 (S556)

ConjugateUnconjugated

Other NamesATG1; ATG1A; hATG1; ULK1; UNC51;

Accession NoO75385

Uniprot O75385

Calculated MWPredicted band size: 113 kDa

Sdspage MWObserved band size: 130

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : ULK1 (Phospho-Ser556) Rabbit mAb at 1/1k dilution
Lane 1 :293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 130
Exposure time: 5 seconds
All lanes : ULK1 (Phospho-Ser556) Rabbit mAb at 1/1k dilution
Lane 1 :NIH/3T3 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 130
Exposure time: 3 seconds
Immunocytochemistry/Immunofluorescence ULK1 (Phospho-Ser556) antibody (14300)

ICC/IF staining of ULK1 (Phospho-Ser556) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14300 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

  Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes.

If you have published an article using product 14300, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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