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Recombinant Rabbit Monoclonal Antibodies > PDPK1 (Phospho-Ser241) Rabbit mAb
PDPK1 (Phospho-Ser241) Rabbit mAb#14320
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All lanes : PDPK1 (Phospho-Ser241) Rabbit mAb at 1/1k dilutionLane 1 : 293 whole cell lysatesLane 2 : 293 treated with 100ng/ml EGF for 15 minutes whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 63 kDa Observed band size: 63 kDaExposure time: 5 seconds
Formalin-fixed, paraffin-embedded human breast tissue stained for PDPK1 (Phospho Ser241) using 14320 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence PDPK1 (Phospho Ser241) antibody (14320)
ICC/IF staining of PDPK1 (Phospho Ser241) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14320 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
