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Recombinant Rabbit Monoclonal Antibodies > RPS6 (Phospho-Ser240/Ser244) Rabbit mAb
RPS6 (Phospho-Ser240/Ser244) Rabbit mAb#14322
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All lanes : RPS6 (Phospho-Ser240/Ser244) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 3T3 treated with 10% serum for 30 minutes whole cell lysatesLane 3 : MCF7 whole cell lysatesLane 4 : MCF7 treated with 10% serum for 30 minutes whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 29 kDa Observed band size: 32 kDaExposure time: 7 seconds
Formalin-fixed, paraffin-embedded human brain tissue stained for RPS6 (Phospho-Ser240/Ser244) using 14322 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse brain tissue stained for RPS6 (Phospho-Ser240/Ser244) using 14322 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence RPS6 (Phospho-Ser240/Ser244) antibody (14322)
ICC/IF staining of RPS6 (Phospho-Ser240/Ser244) in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14322 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
