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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Smad2/3 (Phospho-Thr8) Rabbit mAb

Smad2/3 (Phospho-Thr8) Rabbit mAb#14323

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Product NameSmad2/3 (Phospho-Thr8) Rabbit mAb

Clone No.SR4363

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProtein A

ApplicationsWB, ICC/IF

Species ReactivityHuman;Mouse;Rat

SpecificityPhospho-Smad2/3 (T8) Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.

Target NameSmad2/3

Other NamesSMAD2;MADH2;MADR2;Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD3;MADH3;Mothers against decapentaplegic

Accession NoQ15796;P84022

Uniprot Q15796;P84022

Calculated MWPredicted band size: 48 kDa

Sdspage MWObserved band size: 62 kDa

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at -20°C /1 year

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : Smad2/3 (Phospho Thr8) Rabbit mAb at 1/1k dilution
Lane 1 : 293T whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 62 kDa

Exposure time: 12 seconds
Immunocytochemistry/Immunofluorescence Smad2/3 (Phospho-Thr8) antibody (14323)

ICC/IF staining of Smad2/3 (Phospho-Thr8) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14323 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control.
Nuclei were counterstained with DAPI.

If you have published an article using product 14323, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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