Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > Src Family (Phospho-Tyr416) Rabbit mAb

Src Family (Phospho-Tyr416) Rabbit mAb#14324

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameSrc Family (Phospho-Tyr416) Rabbit mAb

Clone No.SR4364

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProtein A

ApplicationsWB, ICC/IF

Species ReactivityHuman;Mouse;Rat

SpecificityPhospho-Src (Y416) Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y416.

Target NameSrc Family

Other NamesSRC;SRC1;Proto-oncogene tyrosine-protein kinase Src;Proto-oncogene c-Src;pp60c-src;p60-Src;ASV;

Accession NoP07947;P07948;P06239;P08631;P12931;P06241

Uniprot P07947;P07948;P06239;P08631;P12931;P06241

Calculated MWPredicted band size: 58-61 kDa

Sdspage MWObserved band size: 60 kDa

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at -20°C /1 year

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : Src Family (Phospho-Tyr416) Rabbit mAb at 1/1k dilution
Lane 1 : COLO 201 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : 3T3 treated with 100ng/mL PDGF for 10 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 58-61 kDa
Observed band size: 60 kDa

Exposure time: 7 seconds
Immunocytochemistry/Immunofluorescence Src Family (Phospho-Tyr416) antibody (14324)

ICC/IF staining of Src Family (Phospho-Tyr416) in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14324 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14324, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors