Product Detail
Product NameGSK3b(Ab-9) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHuman, Mouse
SpecificityThe antibody detects endogenous level of total GSK3b protein, this antibodymay cross-react with the GSK-3a due to high sequence homology.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.7~11 (T-T-S-F-A) derived from Human GSK3b.
Target NameGSK3b
ConjugateUnconjugated
Other NamesFactor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta,
Accession NoSwiss-Prot: P49841
NCBI Protein: NP_001139628.1
Uniprot
P49841
Gene ID
2932;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
Cross D. A, et al. (1995) Nature. 378: 785-789.
If you have published an article using product 21002, please notify us so that we can cite your literature.
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