Product Detail
Product NameRaf1(Ab-259) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Raf1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
Target NameRaf1
ConjugateUnconjugated
Other NamesC-RAF; C-Raf; CRAF
Accession NoSwiss-Prot: P04049
NCBI Protein: NP _002871.1
Uniprot
P04049
Gene ID
5894;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 73kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, K562 and HT29 cells using Raf1(Ab-259) Antibody #21006.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Ab-259) Antibody #21006.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.
Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.
If you have published an article using product 21006, please notify us so that we can cite your literature.
et al,Prognostic Impact of Raf-1 and p-Raf-1 Expressions for Poor Survival Rate in Non-Small Cell Lung Cancer.In Cancer Sci on 2012 Oct by Zhi-Xin Qiu, Lei Wang,et al..PMID:22738312
, (2012),
PMID:
22738312
et al,MicroRNA-146b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury. In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu, et al..PMID: 27400799
, (2016),
PMID:
27400799
Yes
