Product Detail
Product NameMEK2(Ab-394) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total MEK2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.392~396 (P-G-T-P-T) derived from Human MEK-2.
Target NameMEK2
ConjugateUnconjugated
Other NamesERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2
Accession NoSwiss-Prot: P36507
NCBI Protein: NP_109587.1
Uniprot
P36507
Gene ID
5605;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 44kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, HepG2, PC12 and HUVEC cells using MEK-2(Ab-394) Antibody #21008.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Ab-394) Antibody #21008(left) or the same antibody preincubated with blocking peptide(right).
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases
Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.
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