Product Detail
Product NameNFkB-p105/p50(Ab-337) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total NFkB-p105/p50 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.335~339 (R-K-S-D-L) derived from Human NFkB-p105.
Target NameNFkB-p105/p50
ConjugateUnconjugated
Other NamesDNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50
Accession NoSwiss-Prot: P19838
NCBI Protein: NP_001158884.1
Uniprot
P19838
Gene ID
4790;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 50 120 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells using NFkB-p105/p50(Ab-337) Antibody #21017 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p105/p50(Ab-337) Antibody #21017(left) or the same antibody preincubated with blocking peptide(right).
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.
If you have published an article using product 21017, please notify us so that we can cite your literature.
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PMID:
39358475
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