Product Detail
Product NameATF2(Ab-112 or 94) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total ATF2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.110~114 or 92~96 (D-L-S-P-L) derived from Human ATF2.
Target NameATF2
Other NamesCREB2; CREBP1;
Accession NoSwiss-Prot: P15336
NCBI Protein: NP_001871.2
Uniprot
P15336
Gene ID
1386;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 65-75 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MDA-MB-435 cells using ATF2(Ab-112 or 94) Antibody #21033 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Ab-112 or 94) Antibody #21033(left) or the same antibody preincubated with blocking peptide(right).
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.
If you have published an article using product 21033, please notify us so that we can cite your literature.
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