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Estrogen Receptor-a Antibody#21066

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Product Detail

Product NameEstrogen Receptor-a Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB;IHC;IF

Species ReactivityHu Ms Rt

SpecificityERα Polyclonal Antibody detects endogenous levels of ERα protein.

Immunogen TypePeptide-KLH

Immunogen DescThe antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha.

Target NameEstrogen Receptor-a

ConjugateUnconjugated

Other NamesER; ESR; ESR1; ESTR; ESTRA

Accession NoSwiss-Prot: P03372
NCBI Protein: NP_000116.2

Uniprot P03372

Gene ID 2099;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. 

Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a(Ab-106) Antibody #21066 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-106) Antibody #21066(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell.
Immunofluorescence analysis of rat-lung tissue.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.

If you have published an article using product 21066, please notify us so that we can cite your literature.

et al,Analysis of prognostic factors and treatment of anaplastic meningioma in China. In J Clin Neurosci on 2015 Apr by Qing Xie, Yu Zhou, et al..PMID:25744075 , (2015),
PMID: 25744075

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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