Product Detail
Product NameHER2(Ab-1221/1222) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total HER2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.1219~1223/1220~1224 (N-L-Y-Y -W) derived from Human HER2.
Target NameHER2
Other NamesC-erbB-2; ErbB2;
Accession NoSwiss-Prot: P04626
NCBI Protein: NP_001005862.1
Uniprot
P04626
Gene ID
2064;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from SKOV3 and MDA cells using HER2(Ab-1221/1222) Antibody #21071.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1221/1222) Antibody #21071(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using HER2(Ab-1221/1222) Antibody #21071.
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.
Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
If you have published an article using product 21071, please notify us so that we can cite your literature.