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HER2(Ab-1221/1222) Antibody#21071

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Product Detail

Product NameHER2(Ab-1221/1222) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total HER2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.1219~1223/1220~1224 (N-L-Y-Y -W) derived from Human HER2.

Target NameHER2

Other NamesC-erbB-2; ErbB2;

Accession NoSwiss-Prot: P04626
NCBI Protein: NP_001005862.1

Uniprot P04626

Gene ID 2064;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from SKOV3 and MDA cells using HER2(Ab-1221/1222) Antibody #21071.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1221/1222) Antibody #21071(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using HER2(Ab-1221/1222) Antibody #21071.
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.
Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

If you have published an article using product 21071, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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