Product Detail
Product NameHER2(Ab-1248) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total HER2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2.
Target NameHER2
ConjugateUnconjugated
Other NamesC-erbB-2; ErbB2;
Accession NoSwiss-Prot: P04626
NCBI Protein: NP_001005862.1
Uniprot
P04626
Gene ID
2064;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody #21072.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1248) Antibody #21072(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Ab-1248) Antibody #21072.
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002)Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.
If you have published an article using product 21072, please notify us so that we can cite your literature.