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b-Catenin(Ab-33) Antibody#21211

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Product Detail

Product Nameb-Catenin(Ab-33) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total b-Catenin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.31~35 (L-D-S-G-I) derived from Human b-Catenin.

Target Nameb-Catenin

ConjugateUnconjugated

Other NamesCTNNB1; CATNB; CTNB1; CTNNB;

Accession NoSwiss-Prot: P35222
NCBI Protein: NP_001091679.1

Uniprot P35222

Gene ID 1499;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela and HT29 cells using b-Catenin(Ab-33) Antibody #21211.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Ab-33) Antibody #21211(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using b-Catenin(Ab-33) Antibody #21211.
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

If you have published an article using product 21211, please notify us so that we can cite your literature.

et al,Lipopolysaccharide-induced inhibition of connexin43 gap junction communication in astrocytes is mediated by downregulation of caveolin-3. , (2010),
PMID: 20093193
et al,Inactivation of PI3K/AKT signaling inhibits glioma cell growth through modulation of [beta]-catenin-mediated transcription. , (2010),
PMID: 20888802
et al,Inactivation of PI3K/AKT Signaling Inhibits Glioma Cell Growth Through Modulation of 尾-Catenin-Mediated Transcription.In Brain Res on 2010 Dec 17 by Yang Yang, Xiao Yue, et al..PMID:20888802 , (2010),
PMID: 20888802
et al,Urocortin Increased LPS-induced Endothelial Permeability by Regulating the Cadherin-Catenin Complex via Corticotrophin-Releasing Hormone Receptor 2.In J Cell Physiol on 2013 Jun by Rong Wan, Rui Guo,et al..PMID:23168683 , (2013),
PMID: 23168683
et al,Lipopolysaccharide-induced inhibition of connexin43 gap junction communication in astrocytes is mediated by downregulation of caveolin-3. In Int J Biochem Cell Biol on 2010 May by Chih-Kai Liao, Seu-Mei Wang, et al..PMID:20093193 , (2010),
PMID: 20093193
et al,Lysophosphatidic acid modulates the association of PTP1B with N鈥恈adherin/catenin complex in SKOV3 ovarian cancer cells. In Cell Biol Int on 2012 Sep by Ruby Yun-Ju Huang, Chen-Chen Wen, et al..PMID: 22582758 , (2012),
PMID: 22582758
et al,A proteomics analysis of rat liver membrane skeletons: the investigation of actin-and cytokeratin-based protein components. In J Proteome Res on 2010 Jan by Qingsong Wang, Jintang He, et al..PMID: 19354234 , (2010),
PMID: 19354234
More
et al,Inactivation of PI3K/AKT signaling inhibits glioma cell growth through modulation of β-catenin-mediated transcription , (2010),
PMID: 20888802
et al,Role of glycosyltransferase PomGnT1 in glioblastoma progression.In Neuro Oncol on 2015 Feb by Jin Lan, Pin Guo et al..PMID: 25085363 , (2015),
PMID: 25085363
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987 , (2012),
PMID: 23191987
et al,PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa. In Mol Cancer Ther on 2013 Nov by Hua Jiang, Yijun Yang, et al..PMID: 24030633 , (2013),
PMID: 24030633
et al,The pivotal role of SUMO-1-JNK-Tau axis in an in vitro model of oxidative stress counteracted by the protective effect of curcumin. In Biochem Pharmacol on 2020 Aug by Lucia Buccarello, Jessica Dragotto, et al..PMID:32502496 , (2020),
PMID: 32502496

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;