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BIM(Ab-69) Antibody#21280

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Product Detail

Product NameBIM(Ab-69) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total BIM protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.67~71 (P-A-S-P-G) derived from Human BIM.

Target NameBIM

ConjugateUnconjugated

Other NamesBCL2-like protein 11; BCL2L11; BOD; Bcl-2 related ovarian death protein;

Accession NoSwiss-Prot: O43521
NCBI Protein: NP_006529.1

Uniprot O43521

Gene ID 10018;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells using BIM(Ab-69) Antibody #21280.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Ab-69) Antibody #21280(left) or the same antibody preincubated with blocking peptide(right).
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-a1, isoform Bim-a2 and isoform Bim-a3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.

Fukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288
Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793

If you have published an article using product 21280, please notify us so that we can cite your literature.

et al,Elucidating the role of the FoxO3a transcription factor in the IGF-1-induced migration and invasion of uveal melanoma cancer cells.In Biomed Pharmacother on 2016 Dec by Fengxia Yan , Rifang Liao et al..PMID: 27881235 , (2016),
PMID: 27881235
et al,The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. In Commun Biol on 2024 Oct 2 by Nian Li, Bing Yun,et al..PMID:39358475 , (2024),
PMID: 39358475

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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