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LC3 antibody#21400

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Product Detail

Product NameLC3 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total LC3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 112-116 (V-Y-A-S-Q) derived from Human LC3.

Target NameLC3

ConjugateUnconjugated

Other NamesLC3A; ATG8E; MAP1ALC3; MAP1BLC3;

Accession NoSwiss-Prot: Q9H492
NCBI Protein: NP_115903.1

Uniprot Q9H492

Gene ID 84557;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000

Western blot analysis of extract from K-562, Hela, JK and 293 cells using LC3 Antibody #21400
Western blotting analysis using LC3 antibody #21400.

Probably involved in formation of autophagosomal vacuoles (autophagosomes).

If you have published an article using product 21400, please notify us so that we can cite your literature.

et al,PIWI-Interacting RNA-004800 Is Regulated by S1P Receptor Signaling Pathway to Keep Myeloma Cell Survival.In Front Oncol on 2020 Apr 15 by Ma H, Wang H, et al..PMID: 32351883 , (2020),
PMID: 32351883

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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