Product Detail
Product NameMDM2 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous levels of total MDM2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2.
Target NameMDM2
ConjugateUnconjugated
Other NamesHDMX; hdm2;
Accession NoSwiss-Prot: Q00987
NCBI Protein: NP_002383.2
Uniprot
Q00987
Gene ID
4193;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 and Hela cells using MDM2 Antibody #21436.
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .
Haupt, Y. et al. (1997) Nature 387, 296-299.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
If you have published an article using product 21436, please notify us so that we can cite your literature.
et al,Developing Light-Switchable Affibody-Based Inhibitors of Key Interactions in the p53 Network
, (2024),
PMID:
Yes
