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Location： Home > Polyclonal Antibodies > Twist1 Antibody

Twist1 Antibody#21642

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Product Detail

Product NameTwist1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of total Twist1 protein.

Immunogen TypePeptide-KLH

Immunogen DescRecombinant fusion protein of Twist (NP_000465.1).

Target NameTwist1

ConjugateUnconjugated

Other NamesSCS; ACS3; CRS1; BPES2; BPES3

Accession NoSwiss-Prot: Q15672
NCBI Protein: NP_000465.1

Uniprot Q15672

Gene ID 7291;

Calculated MW21kDa

FormulationPBS with 0.05% proclin300, 50% glycerol, pH7.3.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:1000;
IHC 1:50 - 1:200;

Western blot analysis of various lysates, using Twist Rabbit pAb at 1:500 dilution.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Twist Rabbit pAb at dilution of 1:200 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Twist Rabbit pAb at dilution of 1:200 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Mouse lung using Twist Rabbit pAb at dilution of 1:200 (40x lens).

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.Cell 112:169-180(2003) 
"Mutations of the TWIST gene in the Saethre-Chotzen syndrome."el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46(1997)

If you have published an article using product 21642, please notify us so that we can cite your literature.

et al,Metformin reverses stem cell?like HepG2 sphere formation and resistance to sorafenib by attenuating epithelial?mesenchymal transformation.In Mol Med Rep.On 2018 Oct by Feng Y, Guo X et al..PMID:30106125 , (2018),
PMID: 30106125
et al,Wogonoside Inhibits Invasion and Migration Through Suppressing TRAF2/4 Expression in Breast Cancer.In J Exp Clin Cancer Res on 2017 Aug 3 by Yuyuan Yao , Kai Zhao,et al..PMID: 28774312 , (2017),
PMID: 28774312
et al,Honokiol inhibits bladder cancer cell invasion through repressing SRC锟? expression and epithelial鈥憁esenchymal transition.In Oncol Lett on 2017 Oct by Lan Shen, Fang Zhang,et al..PMID: 28943942 , (2017),
PMID: 28943942
et al,SALL4, a novel marker for human gastric carcinogenesis and metastasis.In Oncogene on 2014 Nov 27 by L Zhang , Z Xu et al..PMID:24276240 , (2014),
PMID: 24276240
et al,TBBPA stimulated cell migration of endometrial cancer via the contribution of NOX-generated ROS in lieu of energy metabolism. In J Hazard Mater on 2020 Dec 5 by Huilan Su, Ge Guan, et al..PMID:32569978 , (2020),
PMID: 32569978
et al,Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. In Front Cell Dev Biol on 2020 Nov 25 by Guangying Cui, Huifen Wang, et al..PMID:33324633 , (2020),
PMID: 33324633
et al,The essential role of PRAK in tumor metastasis and its therapeutic potential. In Nat Commun on 2021 Mar 19 by Yuqing Wang, Wei Wang, et al..PMID:33741957 , (2021),
PMID: 33741957
More
et al,Interleukin-6 and Hypoxia Synergistically Promote EMT-Mediated Invasion in Epithelial Ovarian Cancer via the IL-6/STAT3/HIF-1 α Feedback LoopInAnal Cell PatholOn2023 Feb 13byTongshuo Zhang?1?2?3,?Jing Yang et al..PMID:?36814597 , (2023),
PMID: 36814597

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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