Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > EPHB2 Antibody

EPHB2 Antibody#21696

Availability: Yes
Total Amount:$
Product Detail

Product NameEPHB2 Antibody

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total EPHB2 protein.

Immunogen DescPeptide sequence around aa.896~900 (L-S-S-G-I) derived Human from EPHB2.

Target NameEPHB2

ConjugateUnconjugated

Other NamesCAPB; DRT; EK5; EPH receptor B2; Ephrin type-B receptor 2

Accession NoSwiss-Prot: P29323
NCBI Protein: NP_059145.2

Uniprot P29323

Gene ID 2048;

Sdspage MW118kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details

 Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK and A549 cells using EPHB2 Antibody  #21696.

 Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. Beside axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses.

 

Kalo MS, Yu HH, Pasquale EB. J Biol Chem 276, 38940-8 (2001)

If you have published an article using product 21696, please notify us so that we can cite your literature.

et al,EphB2 represents an independent prognostic marker in patients with gastric cancer and promotes tumour cell aggressiveness.In J Cancer on 2020 Feb 21; by Yin J, Li Z, et al..PMID: 32226496 , (2020),
PMID: 32226496

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;