Product Detail
Product NameStrep-tag II Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAffinity chromatography purified via peptide column
ApplicationsELISA WB
Immunogen TypePeptide
Immunogen DescRaised against the amino acid sequence ¡°gggWSHPQFEK¡±.
Target NameStrep-tag II
ConjugateUnconjugated
Other NamesStrep-tag II (IN), StrepII-tag
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Application Details
Western blot analysis of 50ng of StrepII-tagged recombinant protein with StrepII antibody at 1 ug/mL in (A) the absence or (B) the presence of blocking peptide.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
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