Product Detail
Product NameGAPDH Antibody
Host SpeciesChicken
ClonalityPolyclonal
PurificationAffinity chromatography purified via peptide column
ApplicationsELISA WB
Species ReactivityHu Ms Rt
Immunogen TypePeptide
Immunogen DescRaised against a 16 amino acid peptide from near the carboxy terminus of human GAPDH.
Target NameGAPDH
ConjugateUnconjugated
Other NamesGlyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Accession NoP04406
Uniprot
P04406
Gene ID
2597;
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Application Details
Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at 1 ug/mL.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
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PMID:
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