Product Detail
Product NameBeta-actin Antibody
Host SpeciesChicken
ClonalityPolyclonal
PurificationAffinity chromatography purified via peptide column
ApplicationsELISA WB ICC
Species ReactivityHu Ms Rt
Immunogen TypePeptide
Immunogen DescRaised against a 16 amino acid peptide from near the amino terminus of human beta-actin.
Target NameBeta-actin
ConjugateUnconjugated
Other NamesBeta actin, b actin
Accession NoSwiss-Prot:P60709
Gene ID:60
Uniprot
P60709
Gene ID
60;
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Application Details
Western blot analysis of actin in HeLa cell lysate with b-actin antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 2 ug/mL.
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
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