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Bim Monoclonal Antibody#26024

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Product Detail

Product NameBim Monoclonal Antibody

Clone No.mAb (Clone 1C2C8)

Host SpeciesMouse

ClonalityMonoclonal

PurificationImmunoaffinity chromotography purified IgG

ApplicationsELISA WB ICC

Species ReactivityHu Ms

Immunogen TypeRecombinant protein

Immunogen DescRaised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Target NameBim

ConjugateUnconjugated

Other NamesBim (1C2C8): Bcl-2 interacting protein, Bcl-2-related ovarian death gene, BOD, Bcl-2-like 11

Accession NoSwiss-Prot:O43521
Gene ID:10018

Uniprot O43521

Gene ID 10018;

Concentration1mg/ml

FormulationSupplied in PBS containing 0.02% sodium azide.

StorageCan be stored at -20˚C, stable for one year.

Application Details
Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 ug/mL.
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 ug/mL.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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