Product Detail
Product NameKLF4 Monoclonal Antibody
Clone No.mAb (Clone 4E5C3)
Host SpeciesMouse
ClonalityMonoclonal
PurificationKLF4 Monoclonal Antibody is Protein A purified.
ApplicationsELISA WB
Species ReactivityHu Ms Rt
SpecificityAt least three isoforms of KLF4 are known to exist; this antibody will detect all three. KLF4 antibody will not cross-react with other Kruppel-like family members.
Immunogen TypePeptide
Immunogen DescRaised against a 20 amino acid peptide near the carboxy terminus of human KLF4.
Target NameKLF4
ConjugateUnconjugated
Other NamesKruppel-like factor 4, GKLF, EZF
Accession NoSwiss-Prot:O43474
Gene ID:9314
Uniprot
O43474
Gene ID
9314;
Concentration1mg/ml
FormulationSupplied in PBS containing 0.02% sodium azide.
StorageCan be stored at -20˚C, stable for one year.
Application Details
Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
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