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FLUORESCENT MOUNTING MEDIUM#270

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Product Detail

Product NameFLUORESCENT MOUNTING MEDIUM

StorageStore at 4°C Light sensitive

Application Details

1. Apply one drop (approximately 20 μL) of FadeStopTM fluorescent mounting medium onto the coverslip, slide, or chamber slide containing the specimen. The amount of the medium isdetermined by the size of the specimen. As a general rule, apply just enough to cover the specimen area under the coverslip.  2. Seal the coverslip with quick-dry nail polish to prevent leakage of the mounting medium. Apply four drops of nail polish to each side of the coverslip to secure the coverslip. Then, seal all the sides of the coverslip with nail polish.  3. Take images immediately or store the slides at -20°C for up to two months.

Figure 1. Hela cells were stained with CTCF antibody and mounted in FadeStopTM fluorescent mounting medium containing DAPI. The slide was stored at -20°C for 1 month before imaging using a Zeiss LSM 880 confocal laser scanning microscope. A series of scans were performed to assess the photobleaching speed of the fluorescent dye Note that FadeStopTM fluorescent mounting medium effectively protected the fluorescence. Even after 12th scan, the image quality was still high.

If you have published an article using product 270, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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