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P4HA1 Polyclonal Antibody#27468

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Product Detail

Product NameP4HA1 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human P4HA1 (NP_001017962.1).

ConjugateUnconjugated

Other NamesP4HA1;P4HA

Accession NoUniprot:P13674
GeneID:5033

Uniprot P13674

Gene ID 5033

Calculated MW61kDa

Sdspage MW61KDa

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:1000 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using P4HA1 antibody.
Western blot analysis of extracts of Rat skeletal muscle, using P4HA1 antibody.
Immunofluorescence analysis of L929 cells using P4HA1 Rabbit pAb.
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

If you have published an article using product 27468, please notify us so that we can cite your literature.

et al,An algorithm-based meta-analysis of genome- and proteome-wide data identifies a combInation of potential plasma biomarkers for colorectal cancer. In Sci Rep on 2019 Oct 30 by Gawel DR, Lee EJ,et al..PMID:31666584 , (2019),
PMID: 31666584

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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