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Location： Home > Polyclonal Antibodies > Nestin Polyclonal Antibody

Nestin Polyclonal Antibody#29097

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Product Detail

Product NameNestin Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IF,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide corresponding to a sequence within amino acids 1392-1621 of human NES (NP_006608.1).

ConjugateUnconjugated

Other NamesNES;Nbla00170;nestin;Nestin

Accession NoUniprot:P48681
GeneID:10763

Uniprot P48681

Gene ID 10763

Calculated MW177 kDa

Sdspage MW150/177 kDa

Concentration1.0mg/ml

FormulationPBS with 0.09% Sodium azide, 50% glycerol, pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details

WB 1:500 - 1:1000;
IHC 1:50 - 1:200;
IF 1:50 - 1:200

Western blot analysis of lysates from Neuro-2a cells, using Nestin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Nestin Polyclonal Antibody at dilution of 100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Rat brain using Nestin Polyclonal Antibody at dilution of 100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
Immunofluorescence analysis of Neuro-2a cells using Nestin Polyclonal Antibody at dilution of 1:50 (40x lens).

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.

If you have published an article using product 29097, please notify us so that we can cite your literature.

et al,Naoxinqing tablet protects against cerebral ischemic/reperfusion injury by regulating ampkα/NAMPT/SIRT1/PGC-1α pathwayInJ EthnopharmacolOn2024 Mar 25byXiao Sun?1,?Yunfeng Pan et al..PMID:?38159826 , (2023),
PMID: 38159826
et al,Naoxinqing tablet protects against cerebral ischemic/reperfusion injury by regulating ampkα/NAMPT/SIRT1/PGC-1α pathway. In J Ethnopharmacol on 2024 Mar 25 by Xiao Sun, Yunfeng Pan,et al..PMID:38159826 , (2024),
PMID: 38159826

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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