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Anti-His Tag Antibody(DM1), Rabbit mAb#29617

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Product Detail

Product NameAnti-His Tag Antibody(DM1), Rabbit mAb

Clone No.DM1

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationPurified from cell culture supernatant by affinity chromatography

ApplicationsWB, ELISA

Species ReactivityHis tags localized at the amino- or carboxyl?terminus.

Immunogen Desc6X His synthetic peptide coupled to KLH

ConjugateUnconjugated

Other Nameshis tag

Calculated MW70 kDa

FormulationLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

StorageStore at -20˚C for 12 months (Avoid repeated freezing and thawing)

Application Details

WB 1/1000

Western blot analysis of his-tag fusion protein using His-Tag antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000 dilution. His-tag fusion protein : 70kDa, 20ng per lane. Blocking buffer: 5% nonfat dry milk in TBST. Exposure time: 1s.

The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1), Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteinsin bacteria, insect cells, and mammalian cells.

If you have published an article using product 29617, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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